University of Manitoba – Multi-Stress Systemtony
Winnipeg, Manitoba , Canada
|Contact:||Dr. Gary Anderson|
|Equipment:||Three Multi-Stressor Units|
|Temperature:||3.5° to 30° C;|
|Water recirculation level:||99.7% (1 water exchange/5 to 6 days);|
University of Manitoba – Multi-Stress System
A Multi-stressor rack is composed of up to three independent water recirculating systems, one per shelf. Each system includes a Habitat Module (zebrafish tanks & plumbing for water inflow/outflow), and a Filtration Module (biofilter, cartridge filter, heat exchanger, controls) located on an adjacent rack). On each shelf runs a row of LEDs controlled by the Enviro-Monitron.
The Habitat Module is enclosed into opaque insulated cabinet to protect the animals for ambient room lighting and reduce chilling requirements. This unit is made to accommodate hypoxic control, as well as creating normoxic and hyperoxic conditions. The Multi-stressor components are illustrated in the figures below.
University of Manitoba – Multi-Stress System: Flow circulation
The water flow through the unit is divided into two circuits, the Filtration circuit and the Circulation circuit.
From the sump tank, the water is pumped through the 20 microns cartridge filter and the activated carbon filter. From the activated carbon filter, the water flows through the chiller & heater, the biofilter and trickles down back to the sump.
The water coming from the biofilter is collected in the sump and then is pumped directly to the rearing tanks. The effluent from each of the rearing tanks flows along a gutter and then back into the sump tank.
A double hydraulic circuit assures a constant flow to the rearing tanks, regardless of filter clogging.